Identification of a Chemoreceptor for Tricarboxylic Acid Cycle Intermediates

Identification of a chemoreceptor for tricarboxylic acid cycle intermediates: differential chemotactic response towards receptor ligands.

We report the identification of McpS as the specific chemoreceptor for 6 tricarboxylic acid (TCA) cycle intermediates and butyrate in Pseudomonas putida. The analysis of the bacterial mutant deficient in mcpS and complementation assays demonstrate that McpS is the only chemoreceptor of TCA cycle intermediates in the strain under study. TCA cycle intermediates are abundantly present in root exud...

Tricarboxylic Acid Cycle in

Evidence for the occurrence of Permeases for tricarboxylic acid cycle intermediates in Pseudomonas aeruginosa.

Washed suspensions and cell-free extracts of Pseudomonas aeruginosa grown on Lemco agar, to which intermediates of the tricarboxylic acid cycle had been added, were tested for their ability to oxidize succinate, fumarate, malate, pyruvate, acetate, a-oxoglutarate and citrate. Whole organisms had lag periods of 2-3 hr. before citrate was oxidized rapidly, except when citrate or acetate had been ...

Oxidation of Intermediates of the Tricarboxylic Acid Cycle by Extracts of Azotobacter Agile.

Oxidation of fatty acids and tricarboxylic acid cycle intermediates by isolated rat liver mitochondria.

Beginning with the fundamental observation of Warburg in 1913 (1) it has been a general finding that the more highly organized enzyme systems of animal tissues responsible for oxidation of metabolites by molecular oxygen are associated with the insoluble particulate portion of the cell. Among the several approaches which have been used to study the morphology and composition of such catalytical...